Orji Ikechukwu, Ezeifeka George, Amadi Ekperechi Sabinus, Okafor Florence.
Role of enriched media in bacterial isolation from semen and effect of microbial infection on semen quality: A study on 100 infertile men.
Pak J Med Sci Jan ;23(5):885-8.

Objective: The study was undertaken to determine the isolation potential of solid media with and without pre-enrichment as well as the possible influence of microbial infection on semen quality. Methodology: Semen samples of 100 infertile men were cultured on solid media with and without complementation with liquid media culture (pre-enrichment) respectively. The cultured plates were examined macroscopically and the colonies identified. The sperm count, motility and morphological characteristics of the semen samples were also determined. Results: The bacteria recovered from both cultural methods includes Staphylococcus aureus, Staphylococcus epidermidis, ß-haemolytic streptococci, a-haemolytic streptococci, Escherichia coli, Proteus and Klebsiella species. There was a significant increase in bacterial isolation when solid media culture was pre-enriched than when the former was used alone (p<0.05). There was no significant relationship between bacteria isolation and ranges of total sperm count, sperm motility, morphological abnormalities and age groups studied (p<0.05). Conclusion: Pre-enriched solid media is a more efficient technique for optimal bacterial isolation from seminal fluid.

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