Muhammad Saleem, Atiqur Rahman, Nighat Afza.
Efficacy of 0.3% topical Ciprofloxacin and Tobramycin ophthalmic solutions in the treatment of experimental Pseudomonas Aeruginosa keratitis in Rabbits.
Pak J Med Res Jan ;47(2):37-9.

Objective: To compare the efficacy of topical ciprofloxacin 0.3% with tobramycin 0.3% ophthalmic solution for treating the Pseudomonas aeruginosa keratitis in the rabbits. Method: P. aeruginosa was isolated from infected human eye of patients attending Akhtar Eye Hospital, Karachi. Rabbits were obtained from the animal house of Pakistan Council of Scientific & Industrial Research (PCSIR). Experimental bacterial keratitis was induced in rabbits by corneal intrastromal injection. Infection proceeded for 24 hours; all eyes developed corneal ulcer without pretreatment with immunosuppressive agents. Topical ciprofloxacin 0.3% and tobramycin 0.3% ophthalmic solutions were applied in the eyes of test group rabbits and normal saline solutions 0.9% was applied in the eyes of control group rabbits for 72 hours respectively. Corneas were then removed and corneal homogenates were plated with serial dilutions for quantitative bacteriology. Result: Culture results from 20 corneas of the test group rabbits and 6 of the control group were studied. In the test group that was treated with ciprofloxacin, 10 of 10 corneas were sterile while 5 of 10 corneas in the test group II that was treated with tobramycin were sterile after 72 hours of treatment. In control group 6 of 6 corneas were still positive. It is found that ciprofloxacin 0.3% is superior to tobramycin 0.3% solution in treating experimental P. aeruginosa keratitis in rabbit’s model. Conclusion: It is concluded that ciprofloxacin 0.3% solution was superior for P. aeruginosa compared to tobramycin 0.3% solution in treating experimental keratitis in rabbit’s model.

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