Abd El Raheim Mohammed Donia, Saleh Ibrahim Alqasoumi, Amani Shafeek Awaad, Lyle Cracker.
Antioxidant activity of covolvulus hystrix vahl and its chemical constituents.
Pak J Pharm Sci Jan ;24(2):143-7.

Total alcohol and successive extracts (diethyl ether, chloroform, ethyl acetate and n-butanol) of Covolvulus hystrix Vahl were screened for their antioxidant activity using free radical 2-2- diphenyl-1-picrylhydrazyl (DPPH) and Trolox as reference substance. The tested extracts showed variable antioxidant activities. The highest antioxidant activity was achieved in total alcohol (48.32%) followed by ethyl acetate (37. 8 %) and n-butanol (32.96 %) extracts. From the ethyl acetate extract four compounds were isolated and identified as quercetin, quercetin 3-0-rutinoside and quercetin-7-O-rhamnoside in addition to ferulic acid using different spectroscopic analysis such as, 1HNMR, 13CNMR, COSY, HMBC and HMQC.

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