Farzana Yasmeen, Asim Mumtaz, Saleemuz Zaman Adhami, Sajid A Qureshi.
Comparison of cation exchange HPLC and immunoturbidimetric method for determination of hba1c.
Biomedica Jan ;27(2):161-5.

Objective: The objective of this study was to compare and correlate the analytical performance of D10 Hemoglobin Testing System based on Cation Exchange HPLC and Roche Hitachi 902 Immunoturbidimetric method. Subjects and Methods: A total of 110 patients of Type 2 Diabetes Mellitus were included in the study. HbA1c was determined using D-10 Hemoglobin Testing system and Roche Hitachi 902 Analyzer. Results: Both methods showed good correlation with the correlation coefficient (r) of 0.95. Between run Coefficient of variance was found to be lower for HPLC system compared to immunoturbidimtric method. HPLC method also produces a chromatogram that shows the different hemoglobin fractions, allowing identification of different hemoglobin variants. Conclusion: In the present study both methods showed good correlation but the D10 HPLC system provided adequate throughput and improved precision as compared to immunoturbidimetric method.

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