Naveed Muhammad, Muhammad Saeed, Haroon Khan, Sohail Hassan, Farah Gul.
Evaluation of Viola betonicifolia for its nutrition value.
Pak J Pharm Sci Jan ;25(3):639-44.

In the present study, Viola betonicifolia (whole plant) and solvent fractions obtained thereof were evaluated for various micronutrients such as lead, copper, chromium, iron, nickel, zinc, cadmium, cobalt and macronutrients such as sodium, potassium and calcium using atomic absorption spectrophotometer and flame photometry respectively. It was observed that cobalt and cadmium were not detectible in tested samples while remaining nutrients were present in variable concentrations. The concentrations of all metals were compared with the recommended limits for plants and the daily consumption of all the nutrients were calculated on the basis of dose (15 g/70 kg/day or 214 mg/Kg body weight) prescribed by hakims in their practice. The concentration of chromium was far beyond the recommended limits in almost all the samples. Proximate analysis was carried out in the powdered form of the plant. Viola betonicifolia appeared as a good source of life essential nutrients like fats (18.70%), proteins (15.70%), carbohydrates (21.42%), fiber (39.01%) and vitamin C (150 mg/100 g). In conclusion, Viola betonicifolia is an excellent source of various micro and macronutrients for human being and can be used safely as a nutritional supplement.

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