Nahid Tawakal, Naila Tawakal, Mohammad Tahir.
Cyclosporin A Induced Tubulointerstitial Changes in Developing Kidney.
Pak J Med Health Sci Jan ;6(2):392-6.

Purpose: To study tubulointerstitial changes in developing kidney by administering therapeutic doses of CsA to pregnant Albino mice throughout gestation. Materials & methods: Twelve pregnant mice were divided into two groups, A and B, having six animals each. Cyclosporin (Sandimmun, Novartis, Switzerland) was freshly prepared in normal saline daily having a concentration of 1mg/ml and administered subcutaneously by a single dose of 50 mg/kg in the morning to experimental group B during pregnancy from day 0 to day 18. The control group A was treated with the comparable volume of normal saline given subcutaneously for 18 days during gestation. The pregnant mice were sacrificed at the end of experimental period. The fetal kidneys were dissected, fixed in 10% formalin and the sections were stained with Hematoxylin and eosin (H & E) for general histological study, Periodic acid Schiff (PAS) collagen depositions and Masson’s trichrome for demonstration of collagen depositions. These sections were evaluated for interstitial fibrosis and tubular atrophy using Calcineurin inhibitor toxicity (CNIT) Score. Results: Our results clearly indicate that CsA is deleterious for developing kidney in the usual therapeutic doses. The work illustrated harmful effects of Cyclosporin leading to statistically significant histological changes including interstitial fibrosis, tubular atrophy suggestive of fetal nephrotoxicity. Conclusion: The current study investigated the effects of CsA given to mice during gestation in therapeutic doses. The work clearly illustrated harmful effects of Cyclosporin leading to statistically significant histological changes including interstitial fibrosis, tubular atrophy suggestive of fetal nephrotoxicity and may eventually lead to renal failure. It might produce comparable effects in human conceptuses after intake of CsA by pregnant mothers.

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