Danish Javed, Sana Zafar, Khurram Anwar, Mudassar Iqbal Arain, Muhammad Ali Ghoto.
Commensal Bacteria Anginosus Group of Streptococci (AGS) and Cystic Fibrosis Pathogen PS. Aeruginosa in a Biofilmcontent: An in Vitro Study.
J Liaquat Uni Med Health Sci Jan ;14(03):104-9.

OBJECTIVE: The objective of the study was to investigate the application on growth of anginosus group of Streptococci (AGS) by Ps. aeruginosa when grown together, and the effect on the virulence of Ps. aeruginosa when grown together with AGS on a biofilm model. METHODOLOGY: In this study we used nitrocellulose filter paper as biofilm model representing the lung epithelium. The AGS strains used were AGS F (S. constellatus from bronchiectasis), AGS 3a (S. anginosus from cystic fibrosis exacerbation), AGS PC 4890 (S. anginosus from dental plaque), AGS 2227 (S. Intermedius from unknown source), AGS EF 52 (S. contellatus from supra gingival plaque), AGS AC 9612 (S. anginosus from supra gingival dental plaque), AGS AM 699 (S. constellatus from supra gingival dental plaque) and AGS 1a (S. anginosus from cystic fibrosis exacerbation). Ps. aeruginosa strain used was LES 1 (Liverpoolepidemic strain of Ps. seruginosa). RESULTS: A concentration of AGS strain in monoculture ( 51x10cfu), Ps. aeruginosa monoculture ( 51x10cfu), AGS+Ps. aeruginosa mixed culture ( 51x10cfu of each) was used to inoculate the biofilm. NAS agar plate and PIA agar plates were used to count the growth of AGS and Ps. Aeruginosarespectively, and pyocyanin and elastase assays were done to check the virulence of the Ps. aeruginosa. It was observed that there was a 10 folds increase in the growth of all the AGS strains; when grown in co-culture with the Ps. aeruginosa strain as compared to when grown in mono culture; both in the 24 and 48 hours aerobically incubated biofilms. When grown in an anaerobic environment AGS in monoculture had the same growth as when grown aerobically with Ps. aeruginosain co culture. There was no effect in the growth of Ps. aeruginosa in mono or co culture with AGS in 24 and 48 hours biofilm. CONCLUSION: The data supported the hypothesis that anginosus group of Streptococci grows better in presence of Ps. Aeruginosain a co-culture, as compared to when grown alone in an anaerobic environment, whereas the virulence factors ( Pyocyanin and Elsastase production) of Ps. aeruginosa increases when grown in co-culture as compared to when grown alone in aerobic conditions. KEYWORDS: Anginosus group of Streptococci (AGS), Cystic fibrosis, Commensals,Biofilm content.

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