Nuzhat Hassan, Muhammad Zahoor Janjua.
A Morphological study of pancreatic islets in Non-Diabetic and Diabetic rats.
Ann Abbasi Shaheed Hosp Karachi Med Dent Coll Jan ;6:280-7.

To investigate the morphological differences in the pancreatic regions derived from dorsal and ventral primordia of non diabetic and diabetic rats. Light microscopic study of pancreatic islets in normal rats and in rats made diabetic by streptozotocin. Fourteen adult albino rats of JPMC strain were divided into two equal groups. A (non diabetic) and B (diabetic). Diabetes was induced in group B ani mals by a single intraperitoneal injection of streptozotocin 32 mg/Kg body weight. The rats were killed by ether over dose and the pancreas were divided into four regions: lower duodenal derived from ventral lonbe and upper duodenal gastric and splenic derived from dorsal lobe. Sections from each region were stained with H&E and chrome alum haematoxylin and phloxin. In non diabetic rats the splenic region had more numerous and larger islets with greater number of cells while upper duodenal region had fewer and smaller islets. Mean number of cells was directly proportional to the islet diameter. In the lower duodenal region the islets were smaller than those in splenic and gastric regions but significantly larger than those in the upper duodenal region. In the diabetic rats the islets were reduced in size, number and cellular content in all the regions when compared with the corresponding regions of non diabetic rats. Mean number of B cells showed a highly significant decrease (P < 0.001) in all the four regions. Distinct regional differences exist in the pancreatic regions derived from dorsal and ventral lobes and also among the three regions derived from the dorsal lobe in non diabetic rats. In diabetic animals the same regional differences were observed but there was an overall decrease in number and size of the islets and their cellular content.

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