Muhammad Zeeshan Tabassam, Obaidullah Qazi, Zahid Ibrahim, Muhammad Farooq, Muhammad Imam Din.
Detection of Methicilin Resistant (MECA) Gene from Staph Aureus by PCR.
Pak J Med Health Sci Jan ;8(2):432-3.

Aims: To detect mecA gene by polymerase chain reaction (PCR) and to demonstrate an efficient method for the detection of mecA gene of MRSA. Methodology: In the present study, 60 MRSA strains on disc diffusion culture plates were collected from three laboratories from October, 2012 to January, 2013. The DNA of MRSA was extracted by phenol / chloroform extraction method and specimen were processed by the standard PCR technique. Results: The mecA gene was detected in 31 strains from 60 MRSA isolates on disc diffusion. The prevalence of (Mutated?) MRSA strains among MRSA strains on disc diffusion culture plates isolates was 51.7%. These samples give 310 bp products on 1% agarose gel and others 29 samples gave no product. Conclusion: This study shows that the PCR for mecA gene is an efficient method to detect the methicillin resistance S. aureus and mecA gene is necessary for the resistance in methicillin resistance S. aureus.

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