Umair Aslam Shahzad Khan, Muhammad Tahir Khadim, Syed Salman Ali, Muhammad Imran Khan Wajid, Akhter Ali Bajwa, Saba Anjum.
Immunohistochemical expression of p53 in invasive ductal carcinoma of breast in Pakistani women.
Pak Armed Forces Med J Jan ;71(Sup-1):S142-46.

Objective: To determine the frequency of immunohistochemical expression of p53 in invasive ductal carcinoma of breast in Pakistani women. Study Design: Descriptive, cross-sectional study. Place and Duration of Study: Department of Histopathology, Combined Military Hospital, Lahore, from Jul 2015 to Jan 2016. Methodology: This study involved 80 histopathological samples of invasive ductal carcinoma breast which were tested for p53 expression. Sample size with 80 cases was calculated using 95% confidence level and 10% margin of error while expected percentage of p53 expression will be 71.67%. Specimens were selected by non-probability, consecutive sampling. All specimens of newly diagnosed cases of invasive ducal carcinoma at CMH Lahore on histopathological examination of female patients aged between 15-80 years. IHC application of p53 was done as per technique mentioned in the manual given by the DAKO. Results: Patient age was calculated which was between 25 years and 80 years while mean age was 50.76 ± 11.80 years. Majority of the patients were aged between 25-50 years (n=44, 55.0%). In 54 (67.5%) patients lymph nodes were positive for tumor metastasis. Majority 64 (80%) of the tumors were Grade-II followed by Grade-III in 15 (18.7%) patients. The size of the tumor ranged from 0.2 cm to 14 cm with a mean of 4.16 ± 2.59 cm. p53 expression was positive in 38 (47.5%) patients. The expression of p53 was higher in patients who have positive lymph nodes (59.3% vs. 23.1%; p=0.002). However, expression of p53 was not so significant for age (p=0.079), tumor size (p=0.414)......................

PakMediNet -Pakistan's largest Database of Pakistani Medical Journals - http://www.pakmedinet.com