Tahir Aziz Ahmed, Muhammad Mukarram Bashir, Nadeem Ikram, Mohammad Hussain, Shaheen Moin, Zahur Ur Rehman, Karamat Ahmed Karamat.
Lymphocytes and their subsets in Pakistani patients with active Pulmonary Tuberculosis.
Pak J Pathol Jan ;11(4):15-9.

This study was carried out to analyse the various lymphocyte subpopulations in the peripheral blood of patients with active pulmonary tuberculosis. A total of forty adult patients suffering from pulmonary tuberculosis having positive sputum smears for AFB were included in the study; 19 males and 21 females. The control group consisted of 19 males and 14 females (n=33). Peripheral blood samples from the patients and controls were collected aseptically in EDTA containers. A panel of monoclonal antibodies to the CD markers of different lymphocyte subsets was used for quantitative analysis of total and activated lymphocyte subpopulations by flowcytometry. Haemoglobin levels, total leucocyte counts, absolute neutrophil counts and absolute lymphocyte counts were also determined by haematology autoanalyser. Peripheral blood analysis of the patients showed anaemia (Hb: 11.8 g/dl), high leucocyte count (10.2x103/ul) and neutrophilia (8.0x103/ul). The number of activated CD8+ T cells (165/ul) and activated fractions of ?b TCR T cells(0.322), CD4+ T cells (0.283) and ?? TCR T cells(0.5) showed a tendency to increase in the active phase of the disease. The results point to the important role of these subpopulations of T lymphocytes in the immune response against Mycobacterium tuberculosis.

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