Imran Khattak, Munawar Aziz Khattak, Yusra Jamil Khattak, Sana Arbab, Momena Rashid, Syed Amjad Shah.
Use of tooth clearing technique to determine root and canal morphology of permanent mandibular third molars in population of peshawar: an in vitro cross-sectional study.
Khyber Med Uni Med J Jan ;13(4):216-21.

OBJECTIVE: To find out number of roots, root-canals and canal configuration in permanent mandibular third molars through tooth clearing technique. METHODS: In this cross-sectional study, 193 extracted human mandibular permanent third molars with completely formed apical foramen and intact roots were collected from both genders treated at dental hospitals in Peshawar, Pakistan from 1st July to 31st December 2019. After collection teeth were visually inspected to count number of roots, followed by access cavity preparation, pulp extirpation and canal staining with black Indian ink. Decalcification was done by placing teeth in nitric acid for 5 days followed by dehydration in ascending concentrations of alcohol. Complete transparency was achieved by immersing teeth in methyl-salicylate for 72 hours. Transparent teeth were inspected again for number of roots and root-canals. RESULTS: Among 193 extracted mandibular third molars, (n=161; 83.4%) had two-roots and (n=24; 12.4%) were single-rooted. Two-canals were present in vast majority (n=142; 73.6%) whereas three and one-canal were seen in (n=37; 19.2%) and (n=13; 6.7%) teeth respectively. Most common type of root canal pattern was Vertucci’s Type-I in mesial-roots (n=79; 63.7%) and distal-roots (n=120; 96.8%). Vertucci’s Type-II and Type-IV were (n=15; 12.1%) and (n=12; 9.7%) in the mesial-roots respectively. Mandibular third molars didn’t present with any configurations that didn’t fullfill Vertucci’s criteria. Correlation between number of roots and root-canals of mandibular third molars was non-significant. CONCLUSION: Two-roots and two-canals were common patterns for mandibular third molars. Mesial and distal roots were predominant in Type-I followed by Type-II and Type-IV Vertucci’s classification.

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