Khuhawar M Y, Rind M A.
High performance liquid Chromatographic determination of Isoniazid, Pyrazinamide and Rifampicin in pharmaceutical preparations.
Pak J Pharm Sci Jan ;11(2):49-54.

Isoniazid (IS) individually or in the presence of pyrazinamide and rifampicin has been determined by high performance liquid chromatography (HPLC) after derivatization with 6-methyl-2-pyridinecarboxaldehyde. Pyrazinamide and rifampicin separate completely from isoniazid derivative and are determined simultaneously. The chromatography is carried out from YMC-ODS column with elution with methanol: water: isopropanol: acetonitrile: sodium acetate (1 mM) with flow rate 1.7 ml/mim and UV detection at 333 nm. The method is applied for the analysis of Isoniazid B.P Rambuzid and Myrene-p tablets.

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