Viroj Wiwanitkit.
Feasibility of PCR test using MSP1 primers for detection of a human intraerythrocyte pathogen, Anaplasma phagocytophilum.
Pak J Med Res Jan ;44(2):68-71.

Objectives: Human granulocytic anaplasmosis is caused by the microorganism Anaplasma phagocytophilum that is transmitted by Ixodes tick bites. In recent years, this disease has become an important concern in transfusion medicine. There are several attempts to develop the diagnostic tools based on molecular microbiology technique. Recently, a new PCR detection tool for animal anaplasmosis using the primer namely MSP1 has been introduced. Methods: A feasibility test on the PCR test for detection of human anaplasmosis, A. marginale str St. Maries, using this primer was performed. Results: PCR amplification showed a band with start position at 154356 and end position at 156575 (Length 2220). Conclusions: It can be shown that the PCR is feasible to produce a specific band to the human strain as well. Therefore, it is feasible to use the PCR method as a tool for screening anaplasmosis in donated blood and blood products.

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