Yalcin Polat, Safinur Yildirim, Yasar Demir, Nazan Demir.
Enzymatic zinc determination in human pancreatic juice.
Pak J Med Res Jan ;44(2):71-4.

Objective: Evaluation of an enzymatic method for determining trace amount of zinc was in human pancreatic juices. Materials and Methods: Bovine carbonic anhydrase was purified from erythrocyte hemolisate by affinity chromatography. Zinc present in active site of enzyme was removed by dialysis against dipicolinic acid and resulting in apo carbonic anhydrase obtained at a ratio of 100%. The activity of the enzyme was determined by the esterase activity on p-nitrophenyl acetate. Standard curve obtained with constant apoCA (3.6x10-5 mol/L) and changing Zn2+ concentrations. Human pancreatic juices were taken from 10 patients and zinc concentrations with enzymatic method were determined using standard curve. For comparison, the same samples were analyzed in atomic absorption. Results: When the results of the AA and apoCA methods were compared, it was found from the r and p values that there is a good correlation between the two methods. Conclusion: The enzymatic method can easily be used in human pancreatic juice zinc determination.

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