Muhammad Tayyab, Aftab Ahmed Somroo, Ghazala Jaffery, Tahira Tasneem, Naseer Ahmed Chaudrhy.
A Comparative Study of Neutrophil Phagocytic Activity with Different Types of Haemodialyser Membranes.
Proceeding Shaikh Zayed Postgrad Med Inst Jan ;14(2):91-8.

Neutrophil phagocytic activity in Chronic Renal Failure (CRF) patients on haemodialysis with Cellulose Acetate Membrane (CAM) and Cuprophane Membrane (CuM) was investigated. Thirty diagnosed cases of CRF and 15 healthy controls were included in the study. Neutrophil phagocytic activity was assessed by determination of percentage Phagocytosis (% P), Phagocytic, Index (PI) and Nitroblue Tetrazolium (NBT) reduction test. In the control group values of %P, PI and NBT reduction (%) were 69.2± 9.23, 1.45±0.39 and 73.73±10 respectively. With CAM the pre-dialysis values of %P and PI were 67.4±9.2 and 1.32±0.26 respectively, which were not significantly different from the postdialysis values, 65.33±8.18 and 1.25±0.43 respectively. Similarly there was no significant difference in NBT reduction (%) after dialysis with CAM, the pre and postdialysis values were 68.8±10.35 and 66.8±9.98 respectively. On comparison these values were not different from the controls. With CuM values of % P and PI before dialysis were 64.86±11.5 and 1.2±0.31 respectively. After dialysis these values were 53.66±9.86 and 0.95±0.27 respectively. Intercomparison of pre and post-dialysis values of %P and PI showed a significant decrease after haemodialysis with CuM (p < 0.01 and p < 0.05 respectively). When compared with the control group there was again a significant decrease in both % P and PI after dialysis with CuM (p < 0.001). The pre- and post-dialysis values of NBT reduction (%) with CuM were 63.64 f 12.6 and 50.86± 11.9 respectively. Comparison showed a significant decrease after dialysis (p < 0.01). Both the values were significantly lower than the values in the control group (p < 0.05 and P< 0.001 respectively). Comparison of post-dialysis values of % P and PI with CAM and CuM also showed a marked reduction with CuM (p < 0.001 and p < 0.05 respectively). Similarly comparison of post-haemodialysis NBT reduction (%) values between CAM and CuM showed a statistically significant decrease (66.8±9.98 and 50.86±11.9 respectively with p < 0.001). It is concluded that neutrophil function is impaired with use of CuM for haemodialysis.

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