Abdus Samad, Muhammad Asghar, Muhammad Naeem, Noor Rehman, Naheed Asghar, Muhammad Haroon, Safia Rahman, Sadiq Azam, Resham Ali, Aaqib Khan, Ghadir Ali, Ibrar Khan, Farman Ullah.
Identification of pathogenic bacteria isolated from tissues, bones infections and their antibiotic susceptibility pattern at Khyber Teaching Hospital, Peshawar.
Pak J Surg Nov ;35(1):21-5.

Objectives: To determine the frequency of SSIs causing pathogenic bacteria and their antibiotics susceptibility pattern in order to develop a drug of choice to treat SSIs (Tissues and bone infection). Study design: Cross-section study Place and duration of study: This Cross-section study was designed in the Orthopedics unit and Department of Pathology at Khyber Teaching Hospital, Peshawar from May to November 2017. Material and Methods: In this study a total of 433 patients with closed fractures of different age groups of both gender were selected. Samples were collected with sterile swabs and then cultured on different Media like Blood Agar, Mac Conkey Agar and Mannitol Salt Agar. Colonies were Gram stained for the identification of Gram positive and Gram-negative bacteria. Biochemical tests were performed to confirm the clinical bacterial isolates. Antibiotic sensitivity was conducted by ?disc-diffusion? method on Mueller Hinton Agar using CLSI guidelines. Results: Out of 433 cases of SSIs 27 (6.23%) yielded bacterial growth. Out of 27 bacterial infections; (66.66%) were deep and (33.33%) superficial infections. The increase prevalence (51.8 %) of Staphylococcus aureus observed among pathogenic micro-organism, followed by Escherichia Coli (25.9%), Pseudomonas aeruginosa(11.11%), Enterobacter spp(7.4%) and Salmonella spp(3.75 %). S.aureus showed good response to Gentamycin (CN), Clindamycin (DA), Fusidic Acid (FD), Vancomycin (VAN) and Amikacin (AK) and resistant to Ampicilin (AMP), Ciprocin (CIP), Velocef (CE) and Amoxicilin (AMC). The E.coli was found to be sensitive to Amikacin (AK), Imipenem (IMP), Sulzone (SCF) and Cefoxitin (FOX) while resistant to Ampicilin (AMP), Fosfomycin (FOS), Levofl oxacin (Lev) and Amoxicilin (AMC). Conclusions: We observed a high rate of infection in our study as compare to the previous studies. We also concluded that in SSIs, other micro-organisms are also involved including Pseudomonas spp, Enterobacter spp and Salmonella spp.

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