Saeed Ahmed Shaikh, Abdul Hameed Khan, Qamar Aziz, Saiful Haque, Khairunnisa Saeed.
Evaluation of relaxant effect of Levcromakalim on oxytocin induced contraction of isolated Rat uterus.
Proceeding Shaikh Zayed Postgrad Med Inst Jan ;19(2):77-81.

Objective: To observe the relaxant effect of Levcromakalim (a Potassium Channel Opener) on the Oxytocin induced contractions of the non-pregnant, isolated rat uterus. Method: This study was carried out in the Department of Pharmacology, Shaikh Zayed, Post Graduate Medical Institute, Lahore. The Female non-pregnant albino rats (Sprague dawley strain), weighing approximately 150 to 200g were included in the study. The living animals were injected with Diethylstilbestrol (0.1 mg/kg), intramuscularly, 24 hrs before the experiments to make the uterus more sensitive to the effect of Oxytocin. The animals were then sacrificed, the uterus of each animal was dissected out and the isolated uterus was placed in the tissue organ bath, containing Dejalon`s solution which was aerated with 100% oxygen and the temperature of the system was maintained at 32°C. First, the normal activity of the isolated uterus was recorded and then the effect of Oxytocin (contractions) was observed. The dose of Oxytocin (1-5ug) was required t o produce sub-maximal contractions of the uterus. Later on Levcromakalim (a potassium channel opener) relaxant effect was observed. The dose of Levcromakalim (1-5ug) was required to reduce 50% uterine contractions produced by Oxytocin. Results: The concentration (dose) of Oxytocin required to produced the sub-maximal contractions of the rat uterus was (Mean±SEM) 4.360±0.644ug. The dose of Levcromakalim required to decrease 50% contractions of the uterus induced by Oxytocin was (Mean±SEM) 3.041±0.364ug. Conclusion: The study proved that Levcromakalim (a Potassium Channel Opener) is a relaxant of the rat uterus and it can be clinically used in Dysmenorrhea and Pre-mature labour in humans.

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