Tatheer Zahra, Shadab Ahmed Butt, Sajid Ali Mustafavi.
Role of beta carotene on histomorphology of liver in acetaminophen-induced hepatotoxicity in rats.
Pak Armed Forces Med J Jan ;61(2):156-9.

Objective: To study the protective role of beta carotene against acetaminophen-induced hepatotoxicity in rats. Study design: Laboratory based randomized control trial Place and duration of study: Department of Anatomy Army Medical College, Rawalpindi; in collaboration with National Institute of Health (NIH), Islamabad. The study duration was one year from Feb, 2009 to Jan, 2010. Materials and methods: Sixty young adult (4-6 weeks old) Sprague-Dawley rats weighing 180-240 g were randomized into three groups. Control group C was given NIH laboratory diet, experimental group A was given toxic dose of acetaminophen 700 mg/kg body weight once daily and experimental group B was given beta carotene 30 mg/kg body weight once daily along with 700 mg/kg body weight acetaminophen once daily for one week. Liver specimens were collected 24 hours after the last dose. Five micron thick sections of liver were stained with H&E for histomorphological study. Results: Microscopic examination demonstrated various grades of periportal and spotty necrosis in experimental group A as compared to control group C. In experimental group B, there was significant attenuation (P-value < 0.001) in periportal and spotty necrosis. Conclusion: It was concluded that beta carotene has hepatoprotective role on histomorphology of liver in acetaminophen-induced hepatotoxicity in rats.

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