Mariyah Hidayat, Muhammad Faseeh Nisar, Sheema Farhan Akram, Muhammad Akram Zahid.
Effect of Streptozotocin on the Morphology of Proximal Convoluted Tubules in Albino Rats.
Pak J Med Health Sci Jan ;6(2):298-301.

Aim: To observe the nephrotoxic effect of STZ on the morphology of proximal convoluted tubules (P.C.T) of albino rats for three weeks & eight weeks duration. Study design: Prospective experimental study. Place and duration of study: This study was conducted in Department of Anatomy, Post Graduate Medical Institute, Lahore from February to April, 2012. Material and methods: 45 male albino rats were obtained from the animal house of BMSI and divided into three groups (gps) - control gp A and STZ treated gp B and C, made diabetic by single dose STZ(37mg/Kg b/w).Serum Glucose level of all the rats were measured before the experiment and twice weekly.Gp B animals were sacrificed at the end of 3rd week and gp C animals at the end of 8th week for a detailed morphological examination of the P.C.T in the Periodic Acid Schiff—Haematoxylin(PAS-H) stained sections under the light microscope. Serum creatinine levels were also determined in all the gps for the assessment of renal function. Results: Microscopically, gp B slides showed dilated proximal tubules and some of them appeared to be distorted. Kidney sections of gp C showed signs of tubular necrosis with disrupted brush borders and basement membranes. Serum glucose levels were significantly elevated in both gp B and C. Serum creatinine was not significantly elevated in gp B and increased significantly in gp C. Conclusion: STZ at a dose of (37mg/Kg b/w) is severely nephrotoxic. It produces significant hyperglycaemia leading to oxidative damage.

PakMediNet -Pakistan's largest Database of Pakistani Medical Journals - http://www.pakmedinet.com