Faheemullah Shaikh, Mahesh Kumar Lohano, Irfan Memon.
Pattern of Microbes Associated to Keratitis in Patients Presenting at Liaquat University Hospital.
J Liaquat Uni Med Health Sci May ;12(03):145-50.

OBJECTIVE: To determine & isolate causative organisms in the bacterial keratitis and their sensitivity and resistance to different antibacterial agents. MATERIAL AND METHODS: This was a prospective observational study containing a total of 80 patients (study participants) conducted from March 2011 to October 2012 at Ophthalmology Department of Liaquat University Eye Hospital Hyderabad. After taking informed consent, bacterial isolation procedures were done as: a corneal smear was taken after topical anesthesia, obtained by application of a drop of single dose unit; Proparacaine hydrochloride 0.5% (Alcon, Belgium). A portion of each scrapping was examined microscopically for the presence of bacteria by using Gram staining and the isolated bacteria were tested for their sensitivity and resistance against the different antibiotics. RESULTS: In this study, male were found in the majority and mostly patients were from rural areas. Mostly causative organisms isolated from the bacterial keratitis were Staphylococcus aureus and Staphylococcus epidermidis among the gram positive organisms and Pseudomonas among the gram negative organisms, different types of the antibiotics as Cefazolin, Tobramycin, Ofloxacin, Ciprofloxacin and the Norfloxacin were tried and showed the sensitivity with the percentage of 80%,75.0%,78.75%,73.75% and 72.5%, respectively. CONCLUSION: Mostly causative organisms isolated from the bacterial keratitis were staphylococcus aureus and staphylococcus epidermidis among the gram positive organisms and pseudomonas among the gram negative organisms, different types of the antibiotics as Cefazolin, Tobramycin, Ofloxacin, Ciprofloxacin and the Norfloxacin were tried & showed good response regarding sensitivity.

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