Rabia Masood, Rozinah Jaffar, Nadia Zaib, Ali Raza, Naila Umer.
Oral Cytomorphometry of Smokers and Non Smokers.
J Islamic Int Med Coll Jan ;10(3):219-23.

Objective: The objective of the study was to observe and compare the changes in buccal exfoliated cells between smokers and Nonsmokers. Study Design: Cross sectional comparative study. Place and Duration of Study: Study was carried out at Islamic International Dental College, Islamabad and Post Graduate Medical Institute Lahore. The duration of study was six months i.e 1st September 2013‐1st March 2014. Materials and Methods: Convinient non‐probability sampling technique was used. Quantitative data was obtained. The study groups consisted of 66 subjects divided into two equal groups of smokers S and non‐ smokers M, of ages between 15yrs‐60yrs. Cellular diameter CD, nuclear diameter ND and nuclear to cytoplasmic ratio N/C ratio was assessed in buccal mucosal smears taken from clinically normal mucosa of smokers and normal subjects using exfoliative cytology. SPSS version 17.0 was used for data entry and statistical analysis. ANOVA and post‐hoc tuckey were used for statistical analysis. Results: The mean cellular diameter of smokers and non‐smokers was 54.41± 3.30μm and 43.81±2.01μm respectively. The mean nuclear diameter of smokers and non‐smokers was 12.68± 0.90μm and 9.97± 0.80μm respectively. And the mean N/C ratio of group smokers and non‐smokers was 1: 4.43± 0.38 and 1: 4.42± 0.41 respectively. The ONE WAY ANOVA test showed significant results (p=0.000) for cellular diameter CD, nuclear diameter ND and N/C ratio both, while post hoc tukey test gave highly significant results for CD and N/C ratio i.e p=0.000. Conclusion: Exfoliative cytology and cytomorphometry can help in the early detection of cellular changes as these techniques are easy, non‐invasive and reproducible. Moreover, there is significant cause effect relationship between smoking and variables as nuclear diameter ND and N/C ratio.

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