Uzma Bukhari, Fouzia Lateef, Saba Jamal.
Frequency of Subgroups of Diffuse Large B-Cell Lymphoma by Immunohistochemistry.
J Liaquat Uni Med Health Sci Jan ;14(02):78-82.

OBJECTIVE: To determine the frequency of subgroups (germinal centre and non germinal centre) of Diffuse large B cell lymphoma (DLBCL) in a tertiary care hospital. MATERIALS AND METHODS: It is a descriptive cross sectional study performed in histopathology department, clinical laboratories Dr. Ziauddin Hospital Karachi. 62 cases of Diffuse large B cell lymphoma were collected during fifteen (15) months period from April 2011 to June 2012. All resected specimens and small biopsies were grossed; sections were processed overnight and stained by Hematoxylin and Eosin (H&E) for morphologic evaluation. The panel of lymphoid antibodies included CD20, CD79a, CD3, Ki-67 & Pax5 were used for the diagnosis of DLBCL. Then monoclonal antibodies CD 10, BCL-6 and MUM-1 were applied for subgrouping of DLBCL. RESULTS: In a total of 62 cases of Diffuse large B cell lymphoma, 27 (44%) cases were categorized as germinal centre like subgroup and 35 (56%) were categorized as non germinal centre like subgroup. Patient’s ages ranged from 04 to 95 years. 58% patients were males and 42% were females with male to female ratio1.4:1. 36(58%) cases were extranodal and 26(42%) were nodal lymphoma. Non germinal center like subgrpoup was preponderance in both nodal and extranodal lymphoma as 15(58%) and 20(56%) respectively. CONCLUSION: This study reports an increased number of patients of non germinal centre subgroup of Diffuse large B cell lymphoma. Male to female ratio was 1.4:1. Mean age of patient was 45 years.

PakMediNet -Pakistan's largest Database of Pakistani Medical Journals - http://www.pakmedinet.com