Arqam Bakhtyar Zaki, Muhammad Arif Munir, Soaib Ali Hassan, Imtinan Akram Khan, Uzair Mukhtar, Ali Arsalan, Shomaila Mushtaq.
Adulticidal efficacy of Azadirachta Indica (Neem Tree), Sesamum Indicum (Til) and Pinus Sabinaena (Pine Tree) extracts against Aedes Aegypti under laboratory conditions.
Pak J Med Res Jan ;55(1):11-5.

Background: Emergence of resistance to insecticides against mosquitoes and other disease vectors has prompted the need for alternate sources of mosquito control which are more target-specific, easily bio-degradable and effective against mosquitoes. Materials and Methods: The adulticidal efficacy of crude Neem oil extract, Pine oil extract and Til oil extract with five different concentrations i.e. 2%, 4%, 6%, and 8% was tested against five to six days old sugar fed female Aedes aegypti using standard WHO Bioassay test kit. The percentage mortality was observed after 24 and 48 hours under laboratory condition. Results: The LC50 (Lethal Concentration)values of Neem oil extract against adults of Aedes aegypti were 1.17 ml and 0.09 ml after 24 and 48 hours of exposure respectively, while the LC99 were 4.27 ml and 1.17 ml after 24 and 48 hours of exposure respectively. The LC50 values of Pine oil extract after 24 and 48 hours were 0.25 and, 0.06 ml respectively, while LC99 values at 24 and 48 hour were and 0.89 ml and 0.64 ml respectively. The LC50 values of Til oil extract after 24 and 48 hours of exposure were 1.17 ml and 0.05 ml respectively while LC99 values were 4.18 ml and 1.28 ml after 24 and 48 hours of exposure respectively. The LC50 and LC 99 values were indicative that the plant extracts were highly toxic after 48 hours of exposure. Among the tested plant oil extracts the maximum adulticidal efficacy was observed in the pine oil extract. No mortality was observed in controls. Conclusion: Crude extract of Pine oil was more effective against Aedes aegypti as compared to Neem and Til oil. Key words: Adulticidal activity, plant oil extracts, Aedes aegypti, dengue vector.

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