Azeem Ahmed, Nusrat Jameel, Fasihuddin Ahmed Ansari, Hajra Khatoon.
Multiple antibiotic resistance among Gram Negative Bacteria isolated from Milk in Karachi.
Pak J Pharm Sci Jan ;14(1):25-31.

A total of 100 Gram negative bacteria isolated from milk in Karachi were screened for their resistance to eleven commonly used antibiotics. The concentration of antibiotics used was 100 mg/ml. About 46% were found to resist antibiotics at the concentration of 100 mg/ml giving different patterns. The resistant bacteria were tested for the presence of R plasmids, both transferable and non-transferable, by conjugation, spontaneous segregation and curing with acridine orange and ethidium bromide. Among the resistant bacteria tested, eight were found to carry non-transferable R plasmids.

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