Lubna Siddique, Madiha Imran.
Effect Of Ghrelin Antagonist On Food And Water Intake In Obese And Type 2 Diabetic Mice.
Pak J Physiol Jan ;13(1):39-42.

Background: Ghrelin is the most common orexigenic hormone which acts as starvation signal by acting on hypothalamic neurons. It acts as adipogenic agent. Increased food intake caused by high levels of ghrelin causes increase fat mass. We investigated the possible beneficial role of ghrelin antagonist on food and water intake in obese and type 2 diabetic mice. Methods: This study was carried out at Department of Physiology, Army Medical College, Rawalpindi in collaboration with National Institute of Health, Islamabad, from Jan to Jun 2013. A total of 50 healthy male BALB/c mice were divided into 5 groups. Group I served as control, groups II and III were obese, and group IV and V were diabetic. Group II, III, IV and V were fed high-fat diet for 4 weeks. Group IV and V were given intraperitoneal (IP) injection of streptozotocin to induce type 2 diabetes mellitus. Ghrelin antagonist was injected IP to Group III and V for 6 days. Food and water intake were measured daily. Body weights were measured twice a week and at the end of experiment. Terminal intracardiac blood extraction was done and samples were analyzed for fasting plasma glucose levels. Results: Food intake decreased significantly in group III (obese with Ghrelin antagonist) and group V (diabetic with Ghrelin antagonist) as compared to controls after injection of ghrelin antagonist. Conclusion: Ghrelin antagonist decreases the food and water intake significantly in obese and type 2 diabetic mice.

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