Zafar Saied Saify, Kiran Rafiq.
Characterization of Piperidine against Plasmodium falciparum; A review of Successful Role for managing Widespread Public Health Disaster, Malaria by Piperidine Derivatives.
Ann Jinnah Sindh Med Uni Jan ;1(2):15-9.

The diversified pharmacological distinctiveness have positioned the piperidine moiety in almost all the therapeutic fields with excellent index including malaria. This modification led drug designing to the development of uncountable potent derivatives to inhibit this parasite that is rocking the world towards high fatality rate. The current study reveals the role of piperidine analogues for the management of malaria and that can be further worked for future proposal of new medicinal moieties. Methods: The antimalarial activity of drug molecule is measured through plasmepsin inhibition. For this purpose plasmepsin II and cathepsin D (Biodesign International, USA) assays are measured using a fluorescence resonance energy transfer (FRET) based substrate DABCYL-Glu-Arg-Nle-Phe-Leu-Ser-Phe-Pro-EDANS (malaria FRET-1; Ana Spec Inc., USA). The assay is performed with plasmepsin II/cathepsin D (1.2 nM) and substrate (malaria FRET-1; 1.0 1.IM) in 0.1 M Sodium acetate buffer pH 5.0, containing 10% Glycerol and 0.01% Tween 20. The assays are performed with 5.0% final concentration of DMSO. Conclusion: It is to be concluded that biological and pharmacological behaviors demonstrated by the novel derivatives, proved them more potent than their parent compounds when compared with relative standards. Consequently these active molecules are suggested for further study and to take up them as potent therapeutic agent to be used in future.

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