Noor Mohammad Khaskhely, Motoyoshi Maruno, Atsushi Takamiyagi, Saeef Taher Ramzi, Kimiko Takei, Hiroshi Uezato, Shigeo Nonaka.
Disseminated Superficial Actinic Porokeratosis report of a case with Electron Microscopic observation, Immunohistochemical and Molecular Biological Analysis of P53 gene.
J Pak Assoc Derma Jan ;11(2):51-7.

A 59 year-old male, patient presented with complaint of erythematous papules on his upper extremities since 10 years. Histopathological examination disclosed the presence of column of dyskeratotic cells called coronoid lamella. Ultrastructural examination disclosed intra-epidermal edema below the coronoid lamella. The striking feature in the epidermis was that the keratinocytes contained many cytoplasmic vacuoles. PCNA showed scattered positive staining all over the epidermis. Immunohistochemical examination for p53, tumor suppressor protein showed positive staining more frequently in the epidermis central to the coronoid lamella. On PCR-SSCP examination; present case showed no positive band for p53 gene mutation. It was concluded that the over-expression of p53 protein is not due to p53 gene mutation. The abnormal p53 expression may have some relavance to the damage of epidermal cells that resulted from porokeratosis.

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