Bashir Y, Tahir M, Lone K P.
Effect Of Aqueous Garlic Extract On Androgen Induced Changes In Ovaries Of Prepubertal Female Albino Rats.
Biomedica Jan ;33(2):113-9.

Background and Objectives: Administration of exogenous androgens to immature female rats had been reported to produce polycystic ovaries. The current study is designed to evaluate these effects and to assess the protection provided by aqueous garlic extract (AGE). Methods: Fifty prepubertal female rats of age 21 days were divided into five groups A, B, C, D and E. Group A served as control and received 5 ml/kg/day of propylene glycol subcutaneous for 14 days. Group B received testosterone propionate (TP) 10 mg/kg/day dissolved in 5 ml/kg of propylene glycol subcutaneous for 14 days. Group C received TP 10 mg/kg/day dissolved in 5 ml/kg of propylene glycol subcutaneous and concomitantly AGE 200 mg/kg/day orally for 14 days. Group D received TP 10 mg/ kg/day dissolved in 5 ml/kg of propylene glycol subcutaneous for 14 days and, aqueous garlic extract 200 mg/kg orally from day 14 – 21. Group E received TP 10 mg/kg/day dissolved in 5 ml/kg of propylene glycol subcutaneous for 14 days with no intervention till day 21. Animals of group A, B and C were sacrificed at day 15 and of group D and E at day 22, ovaries were removed and examined. Results: There was no significant difference in gross parameters of the ovaries between control and experimental groups; however weight and volume of ovaries were significantly increased in group B. Histological sections stained with H&E, showed significant increase in number of large cystic and antral follicles in groups B and C respectively; however the number and size of cystic follicles were reduced after treatment with AGE. Conclusion: The results showed that AGE prevents and decreased the number and size of cystic follicles in androgen treated ovaries of immature rats.

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