Ahmad M, Tahir M, Lone K P.
Protective Effect Of Spinacea Oleracea Extract On Cyclosporine A Induced Nephrotoxicity In Male Albino Rats.
Biomedica Jan ;32(4):271-6.

Background and Objective: Cyclosporine A (CsA), is a powerful immunosuppressant that has revolutionised the field of transplantation. CsA causes nephrotoxicity which is characterised by arteriolar hyalinization, shrunken glomeruli and stripped tubular necrosis. Spinach (Spinacea oleracea) has an antioxidant and anti-mutagenic potential. The present study was designed to observe the effects of spinach extract against CsA induced histological changes in kidneys. Methods: Twenty eight rats (6 – 8 weeks) were divided into 4 groups; A, B, C and D. Group A served as control and was given 2 ml/kg of olive oil intraperitoneally for 7 days. Group B was given Cyclosporine A, 20 mg/kg dissolved in 2 ml of olive oil intraperitoneally for 7 days. Group C was given 0.5 ml of 1% nhexane extract of Spinacea oleracea for 14 days concomitantly with Cyclosporine A, 20 mg/kg dissolved in 2 ml of olive oil intraperitoneally from day 7 to 14. Group D was given 0.5 ml of 1% n-hexane extract of Spinacea oleracea orally for 14 days. Results: Histological sections of kidneys of CsA treated rats showed extensive vacuolation, necrosis and loss of brush border in tubular cells, peri-tubular infiltration and vascular congestion. There was decrease in mean glomerular diameter. Concomitant administration of Spinacea Oleracea (SO) extract with CsA, significantly ameliorated all these renal lesions. Conclusion: The findings of this study suggest that SO extract has protective effect against the CsA induced nephrotoxicity.

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