Tania Mirza, Kiran Qadeer, Iqbal Ahmad, Hussain Sheikh.
Biochemical Functions and Pharmaceutical and Clinical Analysis of Nicotinamide.
Baqai J Health Sci Jan ;13(2):41-5.

The major biochemical role of nicotinamide (vitamin B3) is its involvement in redox reactions and energy metabolism. The biological forms of nicotinamide are nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucleotide phosphate (NADP) coenzymes. Nicotinamide is a component of vitamin B-complex and is used in combination with other vitamin B compounds in pharmaceutical preparations. Several analytical methods have been used for the assay of nicotinamide in commercial products. These methods include high-performance liquid chromatography (HPLC), mass spectrometry in the multiple reaction modes (LC/UV/MS/MRM), liquid chromatographic-isotope detection mass spectrometry (LC/DMS), and planner chromatography-multiple detection by electro-spray mass spectrometry (ES/MS), capillary zone electrophoresis (CZE), thin-layer chromatography (TLC)-densitometry, differential pulse polarography and cyclic voltametry. The clinical analysis of nicotinamide involves the application of liquid chromatography-tandem mass spectrometry (LC-MS/MS), high-performance liquid chromatography (HPLC) and capillary electrochromatgraphy.

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