Mona Humaira, Abdul Raqeeb, Azhar Memon, Haji Khan Khoharo.
Malondialdehyde, Blood Lipids and Antioxidant Activity in Newly Diagnosed Type 2 Diabetics.
J Liaquat Uni Med Health Sci Jan ;15(2):78-82.

OBJECTIVE: To determine malondialdehyde, blood lipids and anti oxidants in newly diagnosed type 2 diabetic patients. STUDY DESIGN: Observational, comparative study PLACE & DURATION: Department of Medicine, Liaquat University of Medical and Health Sciences Hospital from February 2013- May 2014. SUBJECTS & METHODS: 97 newly diagnosed type 2 diabetics and 50 healthy controls were selected through non-probability purposive sampling according to inclusion and exclusion criteria. Blood samples were collected after 8-12 hours of fasting. Fasting blood glucose level, lipid and lipoprotein levels were measured by Cobas e411 analyzer. Malondialdehyde (MDA), Superoxide dismutase (SOD) and Glutathione peroxidase (GPX) were measured by Diagnostics kit. Zinc and ascorbic acid were measured by using Centronic GmbH-Germany Kit. Albumin, bilirubin, and uric acid were measured on Hitachi Chemistry analyzer. Data was analyzed on SPSS version 21.0. The significant p-value was taken at ≤ 0.05. RESULTS: MDA, blood lipids and anti oxidant mechanisms showed significant differences between diabetics and healthy controls. Total blood lipids and lipid sub fractions were elevated in diabetics compared to controls. MDA was raised 5.16±0.91 vs. 2.16±0.62 µmol/l in diabetics and controls respectively (p=0.0001). The SOD, GPX, Ascorbic acid, Zinc, albumin, uric acid and bilirubin were reduced in diabetics (p<0.001). CONCLUSION: Diabetes mellitus is characterized by free radical formation, lipid peroxidation, altered blood lipids and reduced anti oxidant mechanisms. An increase in malondialdehyde and reduction of antioxidant mechanisms may contribute to secondary complications.

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