Nomana Mahmood Syed, Kaukab Anjum Qureshi, Humaira Ali.
Effects of Sleep Deprivation on the Area of the Prostatic Acini in Rats and the Protective Effects of Omega 3 Fatty Acids.
J Islamic Int Med Coll Jan ;12(3):148-52.

Objective: To study the protective role of omega 3 fatty acids on the histomorphological changes in the area of the prostatic acini in rats, induced by sleep deprivation. Study Design: Lab based randomized control trial. Place and Duration of Study: The study was conducted at Anatomy Department, Army Medical College, Rawalpindi, in collaboration with National Institute of Health (NIH), Rawalpindi for a duration of one year from th th 11 Nov 2014 to 11 Nov 2015. Materials and Methods: Thirty male Sprague Dawley rats, 3-4 months of age with average weights of 200-300 grams (gm) were divided in three groups each having 10 rats .Group A served as control with standard lab diet and regular sleep -wake cycle. Group B was subjected to sleep deprivation of 16 hours followed by a sleep window of 8 hrs daily for 2 months and group C was administrated with omega 3 fatty acids and was sleep deprived as group B for 2 months. At the end of the experimental period rats were anesthetized and their blood sample was drawn for hormonal assay. They were dissected and the prostate gland was removed and fixed in 10 percent formalin. Five micrometer sections were obtained after tissue processing and stained with haematoxylin and eosin (H&E) for histological study. Results: Microscopic examination revealed that the percentage area of the prostatic acini was decreased by 100% in group B and 30% in group C. This revealed that the area of the prostatic acini was decreased in the group B as compared to the control group A. Decrease in the acinar area in the experimental group C was not that marked as compared to experimental group B. Conclusion: It is concluded that sleep deprivation has deleterious effects on the area of the prostatic acini and that omega 3 fatty acids has an ameliorative effect on the area of the prostatic acini.

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