Khawaja Zafar Ahmed, Sadaf Naeem, Assra Noor.
Determination of Phenolic Compounds in Callistemon Viminalis L. and Its Role in Amelioration of Hyperglycemia in Alloxan-Induced Diabetic Rats.
Ann Jinnah Sindh Med Uni Jan ;4(1):4-8.

ABSTRACT Objectives: The objective of the present study was to investigate the total contents and antidiabetic potential of Bottle Brush Callistemon viminalis L. leaves in alloxan-induced diabetic rats. Methodology: The phenolic content of aqueous: methanolic extract of C. viminalis leaves were determined by High Performance Liquid Chromatography (HPLC) technique. The hypoglycemic activity of the aqueous: methanolic leaf extract of C. viminalis was investigated in alloxan-induced diabetic rats. For this purpose, aqueous leaf extract of C. viminalis at two different doses?200 mg/kg and 400 mg/kg?was orally administered after induction of diabetes mellitus with 150mg/kg alloxan monohydrate for seven days. Random blood glucose levels were measured before and after treatment of C. viminalis leaf extract for determining the acute hypoglycemic activity. Results: Significant (P<0.001) dose dependent reduced blood glucose levels were achieved after 7 days of 200 and 400 mg/kg/day C. viminalis treatment in diabetic rats. HPLC results also suggested that C. viminalis is a potential source of flavonoidal compounds with antidiabetic activity. Conclusion: It could be deduced from results obtained in this study that methanolic and flavonoid-rich leaf extracts of C. viminalis have antidiabetic potential in alloxan-induced diabetic rats. Key words: Antidiabetic activity, C.viminalis L., flavonoids, alloxan, hypoglycemic effect

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