Yasir Ali Bhatti, Sadia Zia, Haroon Habib, Shoaib Asif, Muzammil Liaquat Ali, Ali Iftikhar, Mudaser Hussain Abbasi.
Bio Surfactants Production by Pseudomonas Aeruginosa using Agricultural Resources.
Pak J Med Health Sci Jan ;12(2):810-3.

Aim: To yield bio surfactants from Pseudomonas aeruginosa on substrate optimization of 0.75g using agricultural resource and to produce Bio surfactants using low cost materials. Study design: Observational type of Descriptive study. Place and duration of study: The present study was piloted at Institute of molecular biology and biotechnology in University of Lahore. Duration of the study was two years. Methods: The 0.75g size of sample taken of inoculum from growing culture of Pseudomonas aeruginosa was isolated and collected from industrial area of District Kasoor and flasks were then placed into an orbital shaker at speed of 120rpm. Sterile screw capped bottles were used to collect the samples, and aseptic measures were taken . For further use, 4oC was the temperature of sample storage . Results: The surface tension was 62.9, 53. 8, 49.8 and 47.6 mN/m at time one, two, three and four days respectively at 37°C- constant temperature and 0.75g with 1ml inoculum size the molasses were used . Conclusion: After optimizing various growth and environmental factors a production of rhamnolipid was achieved.

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