Saiqa Zahoor, Khalid Khan, Muizz Zahoor, Saad Zahoor.
Depletion of Factor Eight (VIII) Level in Fresh Frozen Plasma After One Month Storage.
J Med Sci Jan ;26(4):305-7.

Objective: To evaluate the efficacy and quality of fresh frozen plasma after one month storage. Material and Method: It was a descriptive cross sectional study conducted in blood bank of Hayatabad Medical Complex Hospital Peshawar,Pakistan. In this study a total number of one hundred and thirty nine (Male n= 121, Female n= 18) healthy volunteer blood donors were enrolled. Whole blood (450 ml ± 10%) was collected from all donors into triple packs blood bag (JMS, Germany). The blood components were prepared within 8 hours in a 2-step spin protocol. Whole blood was centrifuged at 4 0C with low spin (2500 rpm for 10 minutes) and platelets rich plasma was transferred into 2nd pack of triple bag followed by a high spin for 10 minutes at 3200 rpm. Platelets poor plasma (PPP) of 2nd pack was transferred into 3rd pack and fresh plasma was stored at -70 0C. Factor VIII assay was performed on freshly prepared FFPs and repeated after 30 days of storage on Stago STA compact Hemostasis analyzer according to manufacturer instructions. Data was analyzed by SPSS-20 and presented as mean ± standard deviation. Results: Total number of 139 healthy blood donors was enrolled in the present study. Majority of blood donors age was <20 years while only 1.4% blood donors were in the age of 60-70 years, with mean age of 31 years. Out of total donors 121 (87.1%) were male while 18 (12.9%) were female. In more than 95 % of day 1st FFP unites, level of factor VIII greater was 50%. On day 30th factor VIII level was decreased by 23% on average in 95 present of unites. Conclusion: There is sufficient levels of relevant coagulation factor activity present in the plasma obtained from whole blood with in 8 hours of collection and stored at -18°C and would be an acceptable product for patients requiring FFP for bleeding disorders for one mont.

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