Sabahat Javaid Butt, Khalid M Butt, Jamshaid Rahmani, Aqib Javaid Butt, Muhammad Qasim Saeed.
Distribution of Her-2/c-erb – B2 Immunostaining in Invasive Car Breast in Mayo Hospital, Lahore.
Pak J Med Health Sci Jan ;12(1):263-7.

Background: Invasive ductal carcinoma is the most frequently encountered tumour of the breast. Her- 2 / Neu (c-erb-B2) oncoprotein has been demonstrated in variety of breast cancers. Its medical importance stems from its widespread over-expression in breast cancer. Over expression and magnification of Her-2 / Neu (c-erb-B2) oncogene in breast cancer patients show an early metastasis, hormonal and chemotherapy resistance along with reduced life span. Aim: To evaluate the immunohistochemical expression of c-erb-B2 in breast tissue biopsies from patients with invasive carcinomas of breast . Methodology: A total of 75 cases of breast cancer were included in this study. Immunohistochemical protocol was based on avidin – biotin peroxidase complex (ABC) technique of immunostaining. Results: The results showed that out of 75 patients, 73.3% displayed no staining, 12% exhibited faint level of staining, 4% manifested moderate level and 10.7% expressed intense or marked immunostaining. The observations were tabulated and arithmetic mean was calculated . Conclusion: IHC, or Immunohistochemistry, is a special staining process performed on fresh or frozen breast cancer tissue removed during biopsy. IHC is used to show whether or not the cancer cells have HER2 / c-erb-B2 receptors and/or hormone receptors on their surface. This information plays a critical role in treatment planning.

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