Hafiz Shoaib Ali, Muhammad Sharaz, Umaira Ahsan.
Prevalence and Antibiogram of Klebsiella Species from urine and tracheal samples from ICU patients of Sheikh Zayed Medical College/Hospital, Rahim Yar Khan.
Pak J Med Health Sci Jan ;12(3):881-4.

Background: Klebsiella is Gram negative, capsulated, non-motile, multi-drug resistant (MDR) bacilli, belonging to the Enterobacteriaceae family. Klebsiella pneumoniae is the most common causes of pneumonia, urinary tract and bloodstream infections in patients in intensive care unit (ICU). It has become an increasingly important etiologic agent of hospital acquited infections in latest years. The main reason for this is development of resistance to several antimicrobial drugs and express ion of virulence factors . Aim : To determine the prevalence and antibiogram of klebsiella spp from urine and tracheal samples of ICU patients. Methods: A total of 100 clinical samples of urine and tracheal secretions were collected from emergency intensive care unit (E-ICU) and neuro-surgical intensive care unit (N-ICU). Tracheal secretions were streaked on two agar i.e. , MacConkey and Blood agar. Urine samples were streaked on a quarter plate of CLED agar. Plates were placed in incubator aerobically at 35 –37°C. The sensitivity testing of all clinical isolates was done by the standard method. Results: In present study, out of 100 samples, 36% were klebsiella isolates, 24% showed other organisms and 40% showed no growth. 92% of klebseilla spp. isolated from various clinical samples was not sensitive to amoxicillin clavulanic acid followed by gentamycin (64%), piperacillin tazobactam (89%), ciprofloxacin (39%), ceftazidime (100%) and amikacin (39%) and 94% of isolates have growth inhibited to imipenem. Conclusion: it is critically important to have strict antibiotic policies to prevent the spread of the MDR bacteria. Different surveillance programs for multidrug resistant organisms and infection control procedures should be implemented.

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