Muhammad Ikram Ullah, Nazish Mehmood Aisha, Muhammad Shakil, Tayyaba Samreen, Asma Idrees, Shabbir Hussain, Irfan Shaukat.
Genetic Association of AGTP olymorphism in patients with Dilated Cardiomyopathy from Punjabi population of Pakistan.
Pak J Med Health Sci Jan ;13(1):157-60.

Background: Dilated cardiomyopathy (DCM) is a myocardial disease due to ventricular dilatation and systolic dysfunction. The angiotensinogen (AGT) gene mediatesthe protein angiotensinogen which is further cleaved by different enzymes to produce active enzyme angiotensin-II. The protein affects the contractile activity and heart rate and can play role in hypertension pathogenicity. Aim: To detect AGT gene polymorphisms in Dilated Cardiomyopathy patients from Punjabi population. Methods: A retrospective case-control study was conducted on 35 patients diagnosed for DCM cardiomyopathy and 42 ethnically-matched healthy controls. Genotype of AGT was carried out in patients and controls by DNA sequencing following the PCR amplification of specific oligonucleotide sequence. The information collected from each patient was age, gender, hypertension, the age of onset, left ventricular ejection fraction (LVEF), left ventricular internal diameter end diastole (LVIDD) and smoking. The whole blood was collected for the genomic DNA extraction and DNA sequencing of the AGT gene was performed. Results: The results demonstrated an association between SNP rs699 (235T>C) and the DCM patients (P <0.006) than the controls. On the other hand, there were no significant associations observed between rs4762(174C>T) and rs11568053 (235G>A) and DCM in patients. Conclusion: The results conclude that genetic polymorphism SNP rs699 (M235T) of the AGT gene might be a higher-risk allele to cause the dilated cardiomyopathy phenotype.

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