Sarah Fatimah, Asad Mahmood, Nadir Ali, Hamid Saeed Malik, Helen Mary Robert, Muhammad Tahir Khadim.
Determination of fetal hemoglobin levels by cord blood sampling and its association with various influencing parameters.
Pak J Pathol Jan ;30(3):79-82.

Objectives:To determine association between fetal hemoglobin levels and various maternal and infants` influencing parameters. Material and Methods:This descriptive, cross-sectional study was conducted in department of hematology, Armed Forces Institute of Pathology (AFIP), Rawalpindi from 30th March to 30th September 2017. Total 200 females (mothers) of age 20-45 years with gestational age of 25-45 weeks were included. Known parents with persistent fetal hemoglobin (Hb F) and both parents with beta thalassemia trait were excluded. The blood samples were taken under aseptic conditions from the umbilical cord of the fetus. The fetal hemoglobin levels were measured by HPLC (high performance liquid chromatography) (Bio Rad D10). Results:Mean age of females was 29.26 +- 6.07 (+-1 SD) years. Mean gestational age was 32.61 +- 4.09 (+-1 SD) weeks. Out of these 200 infants, 129 (64.50 %) were male and 71 (35.50 %) were females, with male to female ratio of 1.8 : 1. Mean hemoglobin of mother was 11.01 +- 0.607 g/dl. Mean weight of infants was 3.32 +- 1.49 kg. Mean levels of fetal hemoglobin in infants from cord blood sample was 75.35 % +- 11.59. Conclusions:This study showed no significant association between fetal haemoglobin levels in infants with age, haemoglobin level socioeconomic status of mother and also weight and gestational age of infant.

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