Shazia Taj, Shahina Yasmin, Rabia Mubariz, Tariq Butt, Abdul Bari, Uzma Musarat.
Antibacterial Susceptibility Pattern of Gram-Negative ESKAPE Pathogens Isolated from Hospitalized patients.
J Islamic Int Med Coll Jan ;15(4):231-5.

Objective: To determine in-vitro antimicrobial susceptibility pattern of multidrug resistant (MDR) strains of Gram-negative ESKAPE pathogens using VITEK 2 Compact system. Study Design: Descriptive Cross sectional study. Place and Duration of Study: The study was conducted at Pakistan Railway hospital (PRH) Rawalpindi in collaboration with Armed Forces Institute of Pathology (AFIP) Rawalpindi from 1 September 2018 to 1 September 2019. Material and Methods: A total of 320 clinical specimens were collected. The samples included urine, blood, pus, wound, effusions, C.S.F and sputum. After processing the isolates by standard microbiological methods, the antibiotic susceptibility pattern was carried out using VITEK 2 Compact system. Results: A total of 190 clinical isolates of Gram-negative ESKAPE pathogens isolated from 320 clinical specimens. Among them 150 MDR Gram negative ESKAPE pathogens were detected. Out of 190 Gram-negative ESKAPE pathogens, (30.5%) were members of the family Enterobacteriaceae and (69.4%) were from non Enterobacteriaceae. The commonest isolated organism was Acinetobacter baumannii, 36% followed by Pseudomonas aeruginosa, 33.7%, Klebsiella pneumoniae, 26.3% and Enterobacter spp. 4.2%. From all isolates 88% of Klebsiella pneumoniae, 86.9% of Acinetobacter baumannii and 73.4% of Pseudomonas aeruginosa were found MDR. Conclusion: Rapid identification and susceptibility testing of Gram-negative ESKAPE pathogens by VITEK-2 compact system helps in reducing total consumption of antibiotics. MDR was observed in majority of Gramn egative ESKAPE pathogens except for Enterobacter spp. These pathogens revealed comparatively better susceptibility against Minocycline, Tigecycline, and Colistin.

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