Amir Zaman Khan, Shah Zaman, Sardar Muhammad, Riaz Nasim, Mohsina Haq, Muhammad Luqman.
Synergistic effect of Ceftriaxone and diclofenac against Escherichia Coli.
J Saidu Med Col Jan ;11(1):50-5.

Background: The emergence of resistant strains of bacteria has rendered many useful antibiotics ineffective. The researchers are forced to try combination of two or more antibiotics, or addition of a non- antibacterial to them. Ceftriaxone, a third-generation cephalosporin agent is used to treat many infectious diseases, especially those caused by members of Enterobacteriaceae family. We tested the efficacy of ceftriaxone, alone and in combination with various concentrations of diclofenac sodium, against Escherichia coli. Objective: To study the synergistic effect of ceftriaxone and its combination with non-antibiotic drug diclofenac sodium against Escherichia coli ATCC 25922 isolate. Material and Methods: Agar well diffusion technique was applied. 30 ul of solutions of ceftriaxone, and its combination with different concentrations of diclofenac sodium and DMSO were transferred aseptically into the wells. Agar plates were placed in Results: Ceftriaxone 30ug formed a zone of inhibition of 35 mm. Its combination with 25ug and 50ug of diclofenac sodium formed zone of inhibition of 37.5mm while its combination with 100ug diclofenac sodium formed a zone of inhibition of 38.6mm and formed 39.8mm zone of inhibition when combined with 200ug diclofenac sodium. Conclusion: Ceftriaxone activity increased when combined with different concentrations of diclofenac sodium drug showing a synergistic effect of their combination.

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