Ayesha Khalid, Saira Aslam, Ammara Ghafoor, Sania Asif.
Histological Effects of Glutamine on Gentamicin-Induced Nephrotoxicity in Wistar Rats.
J Sharif Med Dent Coll Jan ;08(1):7-12.

Objective: To determine the protective effects of glutamine on gentamicin-induced nephrotoxicity. Methodology: This was a randomized control study which was conducted at the University of Health Sciences, Lahore. Total 36 rats, aged 6-8 weeks with body weights between 170-200 g were divided into four equal groups A to D by random balloting method. Group A was the control group and was given distilled water only by oral gavage for six days. Group B was given gentamicin for six days intraperitoneally. Group C was given a single dose of glutamine by oral gavage and was sacrificed after 24 hours. Group D was given both glutamine and gentamicin intraperitoneally for six days. The animals of groups A, B & D were sacrificed at the end of the experiment on the 7th day and then kidneys were excised from rats and processed for histological examination. Results: Histological parameters of groups A, C, and D showed that proximal convoluted tubules (PCT) and distal convoluted tubules (DCT) of the kidney appeared normal. There were no signs of necrosis in epithelial lining, broken basement membrane as well as disrupted brush borders. In group B, gentamicin administration resulted in the deformation of glomeruli with thickened basement membrane and widening of urinary space. Also, degenerative changes were seen in PCT in the gentamicin group. Conclusion: The nephrotoxicity and the oxidative damage caused by gentamicin on the kidneys of rats were incredibly significant. The use of glutamine supplements had shown promising results in providing protective effects on kidneys in the presence of gentamicin in rats. Keywords: Glutamine. Gentamicin. Rats.

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