Muhammad Rizwan, Sohaib Farooq, Muhammad Salman, Iram Nazir, Asifa Ghazi.
Comparative Analysis of Storage Lesion of Cord Blood and Adult Homologous Blood; a Base to Establish Cord Blood Banks in Pakistan.
Esculapio J Services Inst Med Sci Jan ;15(3):264-8.

Objective: To evaluate the feasibility of collecting Umbilical Cord Blood (UCB) and its efficacy for autologous transfusion to infants by measuring collected volume and by determining storage related changes in comparison with Homologous Adult Blood (HAB). Methods: We collected 50 UCB & 50 HAB units on basis of inclusion criteria. Blood volume was calculated for each unit, microbiological cultures were done on 7th day. Samples from each unit were obtained on Days 0, 15 & 30 to be evaluated for hemoglobin, cell counts, hematocrit, hemolysis rate, potassium levels & Red cell ATP levels. Results: Volume of HAB units was 470+-15 ml while that of UCB was 70+-25 ml. Bacterial culture were +ve in 2 HAB units (4%) & 5 UCB units (10%). In UCB units; Hb, RBC & K+ levels increased while WBC, PLT counts & ATP levels decreased during standardized storage of 30 days. All these findings were comparable & statistically non-significant to HAB changes except K+ levels which were significantly more in HAB. Checked by paired Student t test, p-value > 0.05. The changes were in permissible limits for transfusion. Conclusions: The collected volume of UCB was sufficient for at least two transfusions. Provided the collection technique is more sterile, UCB can be collected & stored for at least 30 days in our health-care settings.

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