Khuram Ashfaq, Muhammad Anees-ur Rehman, Muhammad Tariq, Nasir Ali.
Assessment of Phenolic Contents, Cytotoxic, and Anti-Oxidant Potential of Euphorbia royleana.
RADS J Pharm Pharmaceut Sci Jan ;10(1):3-9.

Aim and Objective: The study was designed to assess the cytotoxic, phenolic contents and anti-oxidant potential of Euphorbia royleana. Methods: The organic extracts were obtained by extraction of whole dried plant with n-hexane, dichloromethane and methanol. Pytochemical screening was carried out by using standard methods. Total phenolics contents were measured by using the Folin-CioEcalteu reagent method. Cytotoxic action was evaluated by brine shrimp lethality bioassay. DPPH radical assay was employed for anti-oxidant activity. Results: Phytochemical investigations confirmed the presence of anthraquinones, saponins, cardiac glycosides and flavonoids. Highest phenolic content (276.41 +- 64 mg GAE/g dry extract wt) was observed in methanol extract. Significant antioxidant potential was exhibited by methanol extract by means of 78.8% inhibition with IC50 value of 35.71ug/ml. In brine shrimp lethality bioassay, methanol extract demonstrated prominent cytotoxicity at highest level of dose with LD50 471.05. Conclusion: The study concluded that Euphorbia royleana contains significant antioxidant and cytotoxic potential. Further investigation is suggested for isolation of secondary metabolites responsible for reported biological activities.

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