Qaiser Hussain Naqvi, Qamar Jamal, Mehmood Hassan Zaidi, Rana Khalid Mehmood, Fozia Abbas.
HER-2/neu over expression; Immunohistochemical determination in invasive lobular Carcinoma of Breast.
Professional Med J Jan ;9(3):213-7.

OBJECTIVE: To determine the frequency of HER-2/neu over expression in invasive lobular carcinoma (ILC) of breast & correlate it with various standard prognostic parameters. DESIGN: This study was performed on formalin fixed paraffin embedded blocks of 18 diagnosed cases of invasive carcinoma of breast with classic lobular pattern. PLACE: Department of Pathology, Basic Medical Science Institute, Jinnah Postgraduate Medical Center, Karachi, Pakistan. METHODS: The paraffin blocks were retrieved and 5m thick sections were cut and stained with H&E for the review of diagnosis and grading. The Immuno Histochemical staining was done on 4 m thick sections by using polyclonal rabbit anti HER-2 ZYMED USA, and ZYMED 2nd generation LAB-SA immunodetection system, to see the HER-2/neu over expression. RESULTS: Sections containing > 50% of tumor cells exhibiting intense circumferential cell membrane staining were scored as positive. The HER-2/neu over expression was seen in 22% of cases. CONCLUSION: The results in this study are statistically insignificant but positive cases can get benefit from Herceptin therapy.

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