Salma Aslam Kundi, Mary Forsling, Halluck Kleimestur, Saleem Wazir, Taj Muhammad Khan.
Effect of Tamoxifen on Pituitary Vasopressin level.
J Ayub Med Coll Abottabad Jan ;16(4):80-3.
Background: Estrogens modulate the release of Arginine Vasopressin (AVP). There is an increased level of AVP in plasma and pituitary under osterogen hormones. Methods: Ten rats were experimental and ten were controls. The ten experimental rats were injected tamoxifen daily subcutaneously for three days in does of 1mg/kg body weight in 0.1 ml vegetable oil base. The ten control rats were given 0.1 ml of vegetable oil vehicle only subcutaneously. On the morning of the fourth day all the twenty rats were decapitated. Blood was centrifuged at 2500 rev/min for 15 min at 4¢XC. Plasma was stored at -20¢XC for assay of AVP. Pituitary eland was removed and homogenized in a test tube containing 1ml of 0.2 molar acetic acid & stored at -20¢XC for analysis of pituitary AVP. Uteri of all rats were removed by careful dissection & weighed. Results: Uterine weight and plasma AVP levels were decreased but the pituitary AVP level was unaffected. Conclusion: Under Tamoxifen (anti-estrogen) effect the plasma vasopressin decreased but the level in the posterior was not affected. The effect of estrogen on Hypothalamo-Pituitary axis (HPA is measurable in the hypothalamus therefore probably anti-estrogenic effect would operate more at hypothalamic level rather than posterior pituitary. This needs further investigation at the Hypothalamus level.
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